Investigation of urinary miRNA profile changes in amphotericin B-induced nephrotoxicity in C57BL/6 mouse, Sprague-Dawley rats and beagle dogs

MicroRNA (miRNAs) have been associated with drug-induced kidney injury (DIKI). However, there are few reports on the utility of miRNAs, when monitoring for nephrotoxicity across multiple species. The purpose of this study was to assess the value of urinary miRNA profile changes as renal safety biomarkers, when monitoring for kidney injury in investigative toxicology studies. To this end, we evaluated urine miRNA expression levels in response to amphotericin B (AmpB-induced nephrotoxicity in mice, rats, and dogs. The results showed that 35 miRNAs were significantly differentially expressed across the three species in response to the induced renal injuries. Dogs showed the highest number of miRNAs with significant changes. miR-205-5p and miR-31-5p were the most consistently altered miRNA biomarkers across all three species. In rodents, these two miRNAs were the most sensitive markers and showed comparable or better sensitivities than the previously published urine protein biomarkers with ..., , , # Investigation of Urinary miRNA Profile Changes in Amphotericin B-Induced Nephrotoxicity in C57BL/6 Mouse, Sprague-Dawley Rats and Beagle Dogs [https://doi.org/10.5061/dryad.c59zw3rk4](https://doi.org/10.5061/dryad.c59zw3rk4) ## Description of the data and file structure The purpose of this study was to assess the value of urinary miRNA profile changes as biomarkers, when monitoring for kidney injury in investigative toxicology studies. To this end, we evaluated urine miRNA response to amphotericin B-induced nephrotoxicity in mice, rats, and dogs. The results showed that there were 35 miRNAs that were significantly differentially expressed across all species in response to the renal injuries, with dogs having the highest number of miRNAs with significant changes. miR-205-5p and miR-31-5p were the most consistent miRNA biomarkers across all 3 species and were also the most sensitive markers in mice and rats. In rodents, these two miRNAs had comparable or better sensitivities than the...