Additional file 5 of qRAT: an R-based stand-alone application for relative expression analysis of RT-qPCR data

Additional file 5. Output of qRAT $$\Delta$$ Δ Cq and $$\Delta \Delta$$ Δ Δ Cq methods using multiple plate dataset: Table of mean $$\Delta$$ Δ Cq, $$-\Delta$$ - Δ Cq and relative quantity (RQ) values, where values of the mutant samples (M) and wildtype samples (WT) are normalized to the reference gene and mean $$\Delta \Delta$$ Δ Δ Cq and fold change (FC) values, where M and WT samples are normalized to the reference gene and made relative to the calibrator WT C.