Integrating into dairy goats chromatin accessible sites during lactation promotes transgene expression efficiency

Combining RNA-seq with ATAC-seq, we screened the open chromatin of dairy goat mammary epithelial cells during lactation and then integrated human lysozyme into various open regions using the CRISPR/Cas9 system. A comparison of lysozyme gene expression and integration efficiency showed that chromatin accessibility at target sites was significantly positively correlated with gene insertion efficiency, and the insertion of foreign genes into highly accessible chromatin regions could enhance its expression. Overall design: 3 dry period and 3 lactation period samples for Capra hircus species were sequenced using ATAC-Seq and RNA-seq by the Beijing Genomics institution (Nanjing, China).