Induction of myeloid cell arginase activity by <i>T. brucei</i> PRF.

Arginase activity was determined in myeloid cells from non-infected mice incubated with (A) WT parasites separated or not by an insert, or WT parasite-released factors (PRF), (B) PRF from WT parasites in presence of indicated antibodies and (C) PRF from WT or TbKHC1 KO parasites, or material purified on anti-PRF antibody affinity column, in presence of indicated antibodies. Heat treatment was at 45°C for 30 min. Data are means ± SEM of 3–4 individual mice of one representative from 3 independent experiments (A, B), or of triplicate cultures of one representative from 2 independent experiments (C). * p<0.05 compared to non-stimulated (-) cells.