Sequences of primers used for PCR, sequencing and Real-Time PCR, followed by sequences of a TaqMan probe, shRNAs, and CRNDEP epitopes used in the present study.
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1) Restriction enzyme recognition sites inserted in primer overhangs were underlined.2) shRNA-coding sequences: sense regions were marked with a wavy line, whereas antisense regions were indicated with a double wavy line; sticky ends, specific to BamHI/HindIII digestion, were double-underlined.Sequences of primers used for PCR, sequencing and Real-Time PCR, followed by sequences of a TaqMan probe, shRNAs, and CRNDEP epitopes used in the present study.
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2015-12-03



