The effect of Massa Medicata Fermentata on intestinal contents in mice with spleen deficiency constipation

Kunming mice were randomly divided into three treatment groups: Normal groups:CC, Model groups:CM, and Massa Medicata Fermentata intervention group:CG. CM and CG were established a spleen deficiency constipation mouse model. After the mold is finished, the CG was infused with 0.15 g/mL Massa Medicata Fermentata water infusion at a dose of 4 g/(kg day), twice a day at 0.4 mL. An equal amount of distilled water was infused in CC and CM for 7 days. After the experiment, 16S rRNA amplicon sequencing was used to detect changes in gut microbiota in the intestinal contents of mice in each experimental group.