CRISPR-BASED GENOME MODIFICATION AND REGULATION
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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summary The present invention provides RNA-derived endonucleases， which are processed for expression in eukaryotic cells or embryos， and methods of using RNA-derived endonucleases for targeted genome modification in eukaryotic cells or embryos. Provides. Fusion proteins are also provided， wherein each fusion protein comprises a CRISPR/Cas-like protein or fragment thereof and an effector domain. The effector domain can be a cleavage domain， an epigenetic modification domain， a transcription activation domain， or a transcription repressor domain. Methods for altering chromosomal sequences or controlling expression of chromosomal sequences using fusion proteins are also provided. The present invention provides RNA-guided endonucleases， which are engineered for expression in eukaryotic cells or embryos， and methods of using the RNA-guided endonuclease for targeted genome modification in in eukaryotic cells or embryos. Also provided are fusion proteins， wherein each fusion protein comprises a CRISPR/Cas-like protein or fragment thereof and an effector domain. The effector domain can be a cleavage domain， an epigenetic modification domain， a transcriptional activation domain， or a transcriptional repressor domain. Also provided are methods for using the fusion proteins to modify a chromosomal sequence or regulate expression of a chromosomal sequence. The present invention provides RNA-guided endonucleases， which are engineered for expression in eukaryotic cells or embryos， and methods of using the RNA-guided endonuclease for targeted genome modification in in eukaryotic cells or embryos. Also provided are fusion proteins， wherein each fusion protein comprises a CRISPR/Cas-like protein or fragment thereof and an effector domain. The effector domain can be a cleavage domain， an epigenetic modification domain， a transcriptional activation domain， or a transcriptional repressor domain. Also provided are methods for using the fusion proteins to modify a chromosomal sequence or regulate expression of a chromosomal sequence.
提供机构：
国家林业和草原科学数据中心
创建时间：
2023-02-12
