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Next Generation Sequencing of RNAs immunoprecipitated with Cyt c under hyperosmotic stress

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE57435
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For the detection of tiRNAs accumulated in Cyt c-IP vs tiRNAs in total cell lysates during hyperosmotic stress in the presence of angiogenin Total cell lysates from mouse embryonic fibroblasts treated with angiogenin under hyperosmotic stress. Lysates split into two parts: one part serveed as the total RNA, the other part served as Cyt c-IP fraction. Small RNAs were enriched from both parts and went through deep sequencing followed by bioinformatic analysis.
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2019-05-15
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