A-to-I editing and adr mutant gene expression in C. elegans germlines

In this study, we performed high-throughput RNA-seq on young adult germline samples from wild-type and adr-2(-) C. elegans hermaphrodites. A-to-I editing sites were identified using SAILOR. Differential gene expression analysis was performed using DESeq2. Overall design: Germlines were isolated from synchronized young adult C. elegans hermaphrodites raised at 20 degrees C. Strains used include N2 and BB20 (adr-2(ok735)). RNA was extracted and poly-A selection was performed prior to library preparation.