Effects of mutating five other P-type ATPase in C. elegans on the elo-5(lf) growth arrest phenotype.

Wild-type and mutant strains for the RNAi feeding experiments were grown from eggs on elo-5(RNAi) and control HT115 plates. Rescue (number of larva past L1 in the second generation) was assayed on the day 6�C7. For the elo-5(gk208) double mutant experiments, worms were grown from eggs on OP50 bacteria supplemented with 1 mM C13ISO. The numbers of larva past L1 larval stage were counted on day 6�C7. In the RNAi feeding experiments with tat-5 RNAi, rescue of the elo-5(gk208) larval lethal phenotype in the first generation was assayed.(a)n = number of plates.(b)no eggs laid by the P0, suggesting a synthetic sterile effect.(c)synthetic lethal in the first generation, tat-6(ok1984) on HT115 alone is 50% embryonic lethal.(d)in the first generation.