Christopher S. Wood, Karl R. Schmitz, Nicholas J. Bessman, Thanuja Gangi Setty, Kathryn M. Ferguson, Christopher G. Burd (2011) CIL:13464, Saccharomyces cerevisiae S288c. CIL. Dataset

Kre2-GFP is not efficiently maintained in the Golgi apparatus when Pik1-mediated PtdIns4P synthesis ceases, implicating Pik1 signaling in retrograde trafficking of Golgi residents. In this image, Kre2-GFP is expressed in the pik1-83 mutant strain at permissive temperature of 26C. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4D 26C pik1-83 panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4D include CIL#s 13460, 13461, 13462, 13463, 13464, 13465, 13466, 13467.

Kre2-GFP在粗面内质网中的维持效率降低，尤其是在Pik1介导的PtdIns4P合成停止的情况下，这一现象暗示了Pik1信号通路在粗面内质网居民物质逆向转运过程中的重要作用。在本图像中，Kre2-GFP在pik1-83突变体菌株的允许温度26°C下表达。细胞在液体培养基中培养后，置于生长培养基中，并使用DeltaVision工作站（Applied Precision）基于倒置显微镜（IX-70；Olympus）和100× NA 1.4油镜在0.4-µm的z轴增量下收集3D图像堆栈。图像在23°C下由12位CCD相机（CoolSnap HQ；Photometrics）捕获，并使用迭代约束算法（Agard，1984年）及测量的点扩散函数进行去卷积处理。图4D中z堆栈中心的某一图像展示了J Cell Biol. 187: 967-975. 2009年的26°C pik1-83面板，图4D包含CIL#s 13460, 13461, 13462, 13463, 13464, 13465, 13466, 13467。