Whole-genome transcriptome profiling of E11.5 Wnt1-Cre;Tgfbr2flfl by microarray of maxilla
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We performed the global gene expression profiling analysis of the maxilla using microarray. Total RNA samples (1 ug per sample) were converted into biotin-labeled cRNA using the GeneChip® IVT Labeling Kit and standard protocols recommended by Affymetrix (Santa Clara, CA). Fragmented cRNA was applied to GeneChip, Mouse Genome 430 2.0 Arrays (Affymetrix) that contain probe sets designed to detect over 39,000 transcripts. Microarrays were hybridized, processed, and scanned, as previously described using manufacturers recommended conditions.
创建时间:
2020-09-09



