Transcriptome analysies reveals the role of OsCBM1 in rice defense against Xanthomonas oryzae pv.oryzae

Carbohydrate-binding malectin/malectin-like domain-containing proteins (CBMs) represent a newly discovered subfamily of lectins that are involved in a range of bio-logical processes across the animal, bacterial, and plant kingdoms. The OsCBM1 gene in rice enhances reactive oxygen species (ROS) burst, in response contributing to drought-stress tolerance. Nonetheless, the functions of OsCBM1 response to biotic stress remain poorly understood. In this study, we found that OsCBM1 was induced by the infection of Xoo and overexpression of OsCBM1 enhances rice resistance to the bacterial blight, while suppression of its expression shows the opposite trend. OsCBM1 may influence resistance to bacterial blight by regulating ROS burst and the SA sig-naling pathway through RNA-seq analysis. Overexpression of OsCBM1 increased SA content and enhanced activities of SOD, POD, and CAT enzymes, whereas knockdown of OsCBM1 exhibited the opposite trend. The expression of genes related to the SA and enzyme activity pathways was validated through qPCR. These results further eluci-date the function of OsCBM1 in biotic stress resistance, providing references for dis-ease-resistant rice breeding. Observing the resistance of WT, OsCBM1-OE, OsCBM1-RNAi to Xoo, and analyze the relevant transcriptome data.