Interplay between mRNA translation and decay in Archaea
收藏DataCite Commons2026-01-26 更新2026-05-03 收录
下载链接：
https://doi.esrf.fr/10.15151/ESRF-ES-2310009316
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资源简介：
Archaea are critical research models for gaining insights into the origin of the eukaryotic cell and the evolution of life. In Eukarya and Bacteria, a general common feature is that mRNA decay and translation are closely coordinated. Proteomic landscapes, direct protein–protein interaction analyses and phylogenomic data support that aRNase J, a 5’-3’ exoribonuclease, engages with a Ski2-like helicase, called ASH-Ski2 and the RNA exosome to potentially exert control over RNA surveillance, at the vicinity of the ribosome.
We recently succeeded in purifying the 30S ribosomal small subunit from P. abyssi in complex with the ASH-Ski2 helicase. A preliminary data collection on our on-site Titan Themis microscope allowed us to identify very encouraging 3D classes. One of the 3D classes refined to 4.5 Å resolution shows the ASH-Ski2 helicase interacting with the 30S subunit at the entry of the tunnel of the mRNA.
Our goal is to obtain high-resolution structures of the 30S:ASH-Ski2 complex.
提供机构：
European Synchrotron Radiation Facility
创建时间：
2026-01-26
