Low pH Solid Phase Amino-Labeling of Complex Peptide Digests with TMTs Improves Peptide Identification Rates for Multiplexed Global Phosphopeptide Analysis

Data from ProteomeXchange, PXD ID: PXD002072. Experiment: ppr140106, file: 20140212_ppr140106_Fr_02a.mzml. From ProteomeXchange: {{i}} We present a novel Tandem Mass Tag Solid Phase Amino Labeling (TMT-SPAL) protocol using reversible immobilization of peptides onto octadecyl-derivatised (C18) solid supports. This method can reduce the number of steps required in complex protocols saving time and potentially reducing sample losses. In our global phosphopeptide profiling workflow (SysQuant) we can cut 24 hours from the protocol while increasing peptide identifications (20%) and reducing side-reactions ... {{/i}}