Additional file 6 of Detection and discovery of plant viruses in soybean by metagenomic sequencing
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Additional file 6 Figure S6. ClYVV-IA-2016 and ClYVV-IA-2017 confirmed by Sanger sequencing in green house grown N. benthamiana, soybean, and broad bean infected plants. Reverse transcription-polymerase chain reaction (RT-PCR) was performed in all 18 samples including mock-inoculated controls. The PCR products were visualized in a 1% agarose gel electrophoresis containing SYBR Safe DNA gel stain. The PCR products were cleaned using ExoSAP-IT PCR product cleanup reagent and sequenced on Sanger sequencing platform. Primer sequences were designed using Primer3 plus from assembled contigs and the primers were used in this study
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Kleczewski, Nathan M.; Sikora, Edward; Whitham, Steven A.; Hajimorad, M. R.; Stewart, Tracey P.; Smith, Damon L.; Mueller, Daren S.; Gaskill, Mikaela A.; Elmore, Manjula G.; Wise, Kiersten A.; Groves, Carol L.
创建时间：
2022-09-14
