Microarray analysis to unravel the function of C-terminus domain of EBF1 by gain-of function approach
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE78842
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To study the function of C-terminus domain of EBF1, a gain-of function microarray analysis was performed by transducing Empty, Ebf1wt and Ebf1DC expressing vectors into Ebf1-/- progenitor cells. Freshly prepared Ebf1−/− c-Kit+ fetal liver cells were transduced with pmys-Empty-IRES-GFP, pmys-EBF1wt-IRES-GFP and pmys-EBF1DC-IRES-GFP. EBF1wt- or EBF1ΔC-transduced cells were sorted as early pro-B cells (GFP+CD19+BP1−Thy1−Mac1−NK1.1−CD3−). vector-transduced cells were sorted as only GFP+.
创建时间:
2017-01-12



