The frequency of courtship behaviors between SB-treated flies and untreated flies.

The frequency of courtship behaviors and successful copulations was measured in pairs where either the male (SB–M) or female (SB–F) was fed 3.0 mM of the antagonist and paired with an untreated partner. The frequency of all early courtship behaviors (orient and wing vibrations) was reduced only slightly, but later behaviors (licking, curling and copulation attempts) was significantly decreased in these pairs when compared to untreated control flies. In both experimental sets, successful copulation was never observed. (n = 10 observed pairs for each behavior, *p<0.001 by Fischer's Exact test).