Mutant p53 alters chromatin accessibility in hematopoietic stem and progenitor cells [ATAC-seq]

To investigate how mutant p53 regulates gene transcription in HSPCs, we performed ATAC-seq assays in LSKs to identify differential regions of chromatin accessibility. We obsreved approximately 600 peaks significantly increased chromatin accessibility in mutant p53 LSKs compared to wild type p53 LSKs. To elucidate the mechanism of how mutant p53 regulates gene transcription in hematopoietic stem an dprogenitor cells (HSPCs), we sorted Lin-Sca1+Kit+ cells LSKs) from the bone marrow of p53+/+ and p53R248W/+ mice and then perfromed ATAC-seq assays.