Y2H analysis for wt and L389S senataxin (1–650).

*Amino acids regions encoded by the representative clones are given for the wild type or mutant interacting clones that were used for subsequent validation retesting: ter = terminus; tot = total protein; and antisense indicates that the BCYRN1 clone fragment was present in the 3′ to 5′ orientation. The number of independent clones obtained from the original wt and mutant library screens are listed as superscript numbers to the+in the ‘wt’ and ‘L389S’ columns, respectively. The SUMO clones (NM_003352) were isoform a, variant transcript 1 (†).