Amplicon_sorter: a tool for reference-free amplicon sorting based on sequence similarity and for building consensus sequences
收藏NIAID Data Ecosystem2026-03-13 收录
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http://datadryad.org/dataset/doi%253A10.5061%252Fdryad.zgmsbccd0
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资源简介：
Oxford Nanopore Technologies (ONT) is a third-generation sequencing technology that is gaining popularity in ecological research for its portable and low-cost sequencing possibilities. Although the technology excels at long-read sequencing, it can also be applied to sequence amplicons. The downside of ONT is the low quality of the raw reads. Hence, generating a high-quality consensus sequence is still a challenge. We present Amplicon_sorter, a tool for reference-free sorting of ONT sequenced amplicons based on their similarity in sequence and length and for building solid consensus sequences.
Methods
Oxford Nanopore sequencing was done on a 9.4.1 MinION Flow cell and basecalling was done in Guppy 4.2.2 (HAC) and Guppy 5.0.7 (supHAC).
创建时间：
2022-05-16
