Analysis of the role of SMARCB1 in bladder cancer cell line T24 [ATAC-seq]

To identify DNA accessibility targets regulated by the SWI/SNF subunit SMARCB1 in bladder cancer, we compared the ATAC-seq signals in T24 cells engineered for SMARCB1 knockout, non-targeting control, or SMARCB1 re-expression following knockout. Analysis of altered DNA accessibility profiles revealed new roles for SMARCB1 in the regulation of gene expression in bladder cancer, and suggested new therapeutic opportunities. Overall design: ATAC-seq analysis of control T24 cells, SMARCB1 knockout T24 cells, and SMARCB1 rescue T24 cells.