Gene expression profile at single cell level of mouse embryonic stem cells (mESCs) cultured in different media pulses. Gene expression profile at single cell level of mouse embryonic stem cells (mESCs) cultured in different media pulses

We used single cell RNA sequencing (scRNA-seq) to analyze gene expression in 6 different mESC pre-culture conditions. Overall design: mESCs and gastruloids were dissociated using TrypLE, dapi-stained and Fluorescence-activated cell (FACS)-sorted into 2 384-well plates per condition, processed according to the CELseq2 protocol and analyzed using scRNAseq.