AZA+atRA reprogramming of head and neck squamous cell carcinoma cell line T-HEp3

We report the RNA expression profile in the AZA+atRA reprogrammed tumorigenic (T-HEp3) HNSCC cell line. T-HEp3 is a human epidermoid carcinoma, which grows on chorioallantoic membrane and metastasizes with high efficiency into the organs of chick embryo. Upon serial passage for more than 40 times in vitro, T-HEp3 loses tumorigenic and metastatic potential and termed as dormant D-HEp3. This is an ideal cell line model system to study cancer dormancy. Overall design: RNA sequencing was performed in T-HEp3 cell line treated (in vitro) with 1. DMSO control, 2. AZA +atRA reprogrammed, 3. NR2F1 knock down DMSO, and 4. NR2F1 knock down AZA + atRA reprogrammed.