mRNA analysis of gene expression in adipose tissue to compare high fat fed sgp130Fc transgenic vs IL6 knock-out vs wild-type mice

We want to analyse on a broad level the difference of gene expression in the adipose tissue between high fat fed sgp130Fc Transgenic which have no IL6 transsignalling and the IL6 knockout which have no IL6 signalling at all. Because the sgp130Tg mice show a marked adipogenic phenotype we want to compare it with the IL6 KO. Total RNA extracted from flash frozen adipose tissue from obese, 5hr-fasted mice.本研究拟从宏观层面探究高脂喂养的两类小鼠的脂肪组织基因表达差异：一类为缺失IL6反式信号转导（IL6 transsignalling）的sgp130Fc转基因（sgp130Fc Transgenic）小鼠，另一类为完全丧失IL6信号转导的IL6基因敲除（IL6 knockout）小鼠。鉴于sgp130Tg小鼠呈现显著的成脂表型（adipogenic phenotype），本研究拟将其与IL6 KO小鼠进行对照分析。实验所用总RNA（total RNA）提取自肥胖且禁食5小时的小鼠的速冻（flash frozen）脂肪组织。