Real-time quantitative PCR analysis of monocytes of major depressed patients [Imipramine study]

Human CD14 positive monocytes were purified from major depressed patients or healthy volunteer frozen PBMC. Patients were treated with imipramin. Responders and non-responders to imipramin were recorded after 6 weeks of treatment qPCR gene expression profiling. Monocytes prior to treatment were used for qPCR analysis. Details of patient selection, monocyte preparation, recording of patient data can be found in Gara Arteaga Henríquez et al, Frontiers of Psychiatry, 2019