Interactions of strigolactone and karrikin signaling pathways on the levels of other phytohormones in rosette leaves of Arabidopsis

To understand the combined role of SL and KAR signaling pathways in regulating levels and metabolism of other plant hormones, we performed the compare transcriptome profiling d14 kai2 and WT plants. Aligent´s whole Arabidopsis Gene Expression Microarray (G2519F-021169, V4, 4x44K) was used. Two-week-old WT and d14 kai2 mutant plants were transferred from GM plates to soil and grown for 10 additional day. The rosette leaves of this 24-d-old seedlings were harvested and frozen in liquid nitrogen immediately. Total RNA was prepared and used for the microarray hybridization. Three biological repeats were used for each plant for transcriptome analysis using the Arabidopsis Oligo 44K DNA microarray (Version 4.0, Agilent Technology).