Soil prokaryotic and eukaryotic diversity in translocated meadows, surrounding area and control meadows. soil metagenome

To combine the conservation of valuable wet meadows with the reclamation of a post-industrial area, 1.3 hectares of turf were translocated from a build-up area to an abandoned quarry. Three of the Molinion meadows were compared four years following their translocation to habitats surrounding translocated meadows and control meadows (remained near the donor site). The composition of the main groups of organisms in the soil: bacteria, archaea, fungi, microfauna and unicellular algae, using a barcoded amplification of the soil DNA followed by Illumina sequencing was performed. Genomic DNA was extracted with the Soil DNA Purification Kit (EURx) using 0.25 g of mixed soil. For the amplification of the prokaryotes, the universal primers 515F and 926R targeting the hypervariable V4-V5 region of the 16S rRNA gene were used with a forward primer tagged. For the detection of the prominent soil eukaryotes, including Fungi, Chlorophyta, Alveolata, Rhizaria, Stramenopila and groups of mesofauna (such as Nematoda, Annelida and Collembola), the PCR targeting ITS2 marker gene was carried out using a mixture of 19 modified ITS3ngs forward primers and a tagged degenerate ITS4ngs reverse primer in line with Tedersoo et al., 2016.