Confocal microscopic images of BMP- 2/4/7 expressions in neurons, oligodendrocytes and astrocytes after agmatine treatment following SCI.

(A) Dual immunofluorescence was done to localize the BMP- 2 expression in neurons (MAP-2) and oligodendrocytes (Olig-2) after SCI. The BMP- 2+/MAP- 2+& BMP- 2+/Olig-2+ cells around the lesion site were higher in the Agm treated group (n = 4) compared with the EC group (n = 4) at7 DPI. Scale bars: 50 µm. (B) The immunostaining of BMP- 7 (red) with MAP-2 (green) showed increased number of BMP- 7+/MAP-2+ cells in the Agm treated group (n = 5) when compared with the EC group (n = 5) at 7 days after SCI. The immunostaining of BMP- 7 with Olig-2 showed an increased number of BMP- 7+/Olig-2+ cells in the Agm treated group (n = 5) compared to EC group (n = 5) at 14 DPI. Scale bars: 10 µm. (C) The immuno co-localization of BMP- 4+ cells in astrocytes (GFAP) showed reduced number of BMP- 4+/GFAP+ cellsaround the lesion site in the Agm treated group (n = 4) compared with the EC group (n = 4) at7 DPI. Whereas, BMP- 4+/Olig-2+ cells were higher in the Agm treated group compared with EC at 35 DPI. The scale bars: 10 µm.