Single Cell Transcriptional Profile of Col1a1GFP+ cells in health and EAE

Col1a1GFP+ cells from the spinal cords of healthy mice or mice in the early stages of EAE symptoms were transcriptionally profiled at the single cell level to determine subtypes of Col1+ CNS cells in health and disease Overall design: Spinal cords from Col1a1GFP mice with or without EAE were dissociated into a single cell suspension, GFP+ cells enriched using FACS sorting and sequenced using the 10x genomics v2 pipeline