Functional analysis of m6A-assisted polyadenylation pathway in Arabidopsis thaliana. Arabidopsis thaliana strain:Col-0

N6-Methyl-Adenosine (m6A) has recently emerged as a prevalent mRNA modification that can affect gene expression in a dynamic and reversible manner in eukaryotes. It is currently believe that m6A deposition occurs co-transcriptionally and is driven by a conserved writer complex composed of methyltransferase-like3 (METTL3/MT-A70), METTL14 (a non-catalytic METTL3-homologous protein), and Wilms Tumor 1-Associated Protein (WTAP/FIP37). Similar to DNA methylation, the biological function of m6A is mediated through the recognition of m6A mark by specific « readers » that predominantly belong to YT521-B homology (YTH) protein family. Studies performed in animals, fungi, and plants have connected m6A-dependent recruitment of YTH-type proteins to the control of various aspects of mRNA metabolism, including stability, splicing, alternative polyadenylation, nuclear export, and translation initiation. More recently, m6A modification has also been implicated in XIST-mediated X-chromosome inactivation and repair of UV-induced DNA damages in mammals, extending the spectrum of m6A activities in animals. Here we reveal the implication of m6A in polyadenylation control and control of genome intergrity in Arabidopsis.