Processed FACS data for "Evolutionarily conserved transcriptional regulators control monoaminergic neuron development"

Contents This dataset contains raw fluorescence-activated cell sorting (FACS) data corresponding to the following samples:QC_Setup, hrs_00_03_Rep1_A1, hrs_00_03_Rep2_A9, hrs_02_05_Rep1_A2, hrs_02_05_Rep2_A10, hrs_04_07_Rep1_A3, hrs_04_07_Rep2_A11, hrs_05_08_Rep1_A4, hrs_05_08_Rep2_A12, hrs_06_10_Rep1_A5, hrs_06_10_Rep2_A13, hrs_09_13_Rep1_A6, hrs_09_13_Rep2_A14, hrs_12_17_Rep1_A7, hrs_12_17_Rep2_A15, hrs_16_22_Rep1_A8, hrs_16_22_Rep2_A16. Cells were sorted on a Beckman Coulter MoFlo Astrios cell sorter (QMC, Nottingham) equipped with a 100 µm nozzle and operated in Purify precision mode using a 1–2 drop envelope to maximise the purity of collected fractions. Single-cell suspensions were maintained at 4 °C throughout sorting by enclosing the flow chamber in a water-cooled housing, thereby minimising heat-induced stress and preserving cell integrity. Sorted samples were collected into a two-way tube holder pre-chilled on ice. Gating strategies were defined using unstained and single-stained controls to exclude autofluorescent events. Broad gates were applied to capture both strongly and weakly fluorescent populations, reflecting the continuum of marker expression. SoxN⁺ and Elav⁺ cells—representing early neurogenic and post-mitotic neuronal cohorts, respectively—were collected together to ensure recovery of the full spectrum of developing neural cells. Data structure and format All files are provided in standard Flow Cytometry Standard (FCS 3.1) format. Keywords FACS, flow cytometry, neurogenesis, monoaminergic neurons, single-cell sorting, developmental biology