Protein final data set.xlsx

Single source white clover (Fabaceae: Trifolium repens) pollen pellets collected by Apis mellifera purchased from Ames Farm (Watertown, Minnesota, USA; www.amesfarm.com) and single source rose (Rosaceae: Rosa spp.) pollen collected by Apis mellifera purchased from the China Health Club (Chongqing, China; www.teaherbalshop.com) were used to create artificial diets to raise C. calcarata eggs. Pollen identity and purity were based on vendor claims. Previously dried and weighed pollen ball samples were sent for nitrogen (N) analysis at Brookside Laboratories, Inc. (New Knoxville, Ohio, USA). Using combustion and thermal conductivity detection in a Carl–Erba total nitrogen analyzer (AOAC International 1996; Gavlak et al., 2003), percent crude protein was calculated by multiplying the %N of the sample by the standard conversion factor of 6.25%. The total protein in the sample was calculated as the percent crude protein times the dry mass of the sample divided by 100.