Transcriptional profiling of macrophages in skin wounds

Macrophages were isolated from wounded skin 4 and 14 days post wounding. Monocytes were adoptively transferred on days 2 and 12 and traced into the wound. Mice were anesthetized by i.p. injection of Ketanest/Rompun. Back skin was shaved and full-thickness excisional wounds were created using a standard biopsy puncher. Mice were housed individually during the entire time course of healing. Monocytes from female B6.RFP mice were isolated from B6.RFP mice via Immuno-magnetic depletion of whole bone marrow, counted and then 3x10^6 cells were adoptively transferred into each previously wounded C57/BL6 recipient via intravenous injection, either 2 or 12 days after injury. Wounds were excised 4 or 14 days after injury. Wounds from several mice were pooled in two separate experiments (2 biological replicates of several mice each).