Gene expression in tissues of root tips from Pinus pinaster seedlings under nitrate nutrition

In the present study, the gene expression changes in four different tissues of root tips from maritime pine were analyzed. The roots were under two nutritional conditions (3 mM KCl or 3 mM NO3K) and harvested after 2 hours of the treatment. This work was funded by Ministerio de Ciencia e Innovación, grant number PID2021-122641NB-C21, MICINN, FEDER, UE. Pine seedlings were subdivided into two groups. The control group was irrigated with 80 mL of 3 mM KCl (C), the second group was supplied with 80 mL of 3 mM NO3K. Root samples were collected 2 hours after the irrigation. Seedling’s root tips were cut and tissue pieces of 5 - 6 mm were imbibed in a specimen holder with Tissue‐Tek optimal cutting temperature embedding medium (Sakura Finetek, Alphen aan den Rijn, The Netherlands) and immediately frozen in liquid nitrogen for cryostat sectioning. The 24 samples were sequenced on an Illumina NovaSeq 6000 as paired-end reads of 150 bp length.