Cimex lectularius nuclear DNA content and chromosome variability

To clarify the origin of the additional chromosomes in Cimex lectularius (human bed bug). Measure different wild populations of C. lectularius with the different cytotype. Test various condition (fresh/frozen/dried/fixed samples, length of freezing, defrosting) of insect genome size FCM measurement and obtain the optimal internal standard for C. lectularius measurements.