Transcriptomic and metabolomic analysis of liver of black seabream (Acanthopagrus schlegelii) fed with Saccharina japonica supplmentation

Macroalgae Saccharina japonica (SJ) has been reported to reduce hepatic lipid accumulation (HLA) in mammals, thus, we hypothesized that dietary SJ can reduce HLA in fish. Firstly, we obtained the transcriptome of the fish by next generation sequencing (NGS) and de novo assbemby. We annotated the unigene of the transcriptome and analyzed differentially expressed genes (DEG) in liver of fish fed with 0, 2.5 and 10% SJ supplementation. Secondly, we identified the metabolite of metabolome by LC/MS analysis and analyzed differentially expressed metabolites (DEM) in liver of fish fed with 0, 2.5 and 10% SJ supplementation. Combined transcriptomic and metabolomic analysis revealed that several important DEG, DEM and co-enriched KEGG pathway were identified to be potentially involved in reducing hepatic lipid accumulation of fish. File S1: DEG in liver of fish between treatments; File S2: DEM in liver of fish between treatments.