Engineered Nanointerfaces for Microfluidic Isolation and Molecular Profiling of Tumor-specific Extracellular Vesicles

Tumor-EVs were captured on a nanostructured interface through immunoaffinity immobilization in a microfluidic channel containing herringbone (HB) structure for efficient mixing (EVHB-Chip). Processing serum and plasma samples from glioblastoma multiforme (GBM) patients, isolated tumor EVs were analyzed using next-generation RNA sequencing analysis, and identified genes specific to glioblastoma as well as transcripts that are hallmarks for the four genetic subtypes of disease (e.g., classical, mesenchymal, neural, and pro-neural). Overall design: Plasma or serum isolated from 13 GBM patients than run through the EVHB-Chip coated with antibodies.(EGFR, EGFRvIII, PDGFR) Isolated Evs analysed using next-generation RNA sequencing analysis.