qF3 Analysis Code

Provided here: -Scripts for Performing quantitative Fast FLIM FRET (qF3) Analysis. Organized into folders by steps (1-13) to run for each biological replicate (Step1_Replicate_Analysis) and then for all combined replicates (Step2_CombineReps_Analysis). -script used to calculate G factor (for which our original data can be provided upon request) -Example Master platemap. -LICENSE file for all code here. -Author: Nehad Hirmiz ([e-mail]) Instructions: -Download all files here and extract .Zip. -Install MATLAB Version R2020a with toolboxes: Signal Processing, Curve Fitting, Image Processing. -IFF starting with INO FLIM Hyperspectral data * then contact lead for INO software package including (Release_r10357 package): INO FHS Acquisition, INO_FHS_Analysis, INO_FHS_Batch Analysis -Follow instructions to run these codes: See associated text at Protocol Exchange: Title: “Automated, quantitative Fast FLIM-FRET (qF3): A step-by-step protocol to measure dissociation constants for protein-protein interactions in live-cell screening applications.” LINK https://doi.org/10.21203/rs.3.pex-1354/v1 And Instructional videos: LINK https://www.youtube.com/playlist?list=PLUiSJrzzg9voe5sjA57oIbfOLAGIrHXRc