Comprehensive analysis of the immune implication of NOTCH1 gene in stomach adenocarcinoma

In order to better understand the relationship between NOTCH1 gene and immunity in gastric adenocarcinoma cells, we performed the silent interference of NOTCH1 gene on three gastric adenocarcinoma cell lines AGS, MKN-45 and MGC-803. We extracted the total RNA from the interfered and untreated blank control cells, and performed lncRNA library construction and sequencing. The results of bioinformatics analysis showed that the silencing of NOTCH1 gene in gastric adenocarcinoma cells activated several immune-related signal transduction pathways, including T cell receptors, chemokines, JAK-STAT, Toll-like receptors, ECM receptors and other signals. Conduction pathway. These results confirm that NOTCH1 may down-regulate the immune response of gastric adenocarcinoma. Overall design: cell lines AGS, MKN-45 and MGC-803( NOTCH1 interfered and untreated blank control) were used for RNA sequencing.