A) Chromatins were prepared from Cdx2-inducible ES cells cultured for 48 - 60 hours in the Dox+ and Dox- conditions. Chromatin immunoprecipitation (ChIP) was carried out by using anti-FLAG M2 affinity
Comprehensive identification and characterization of the binding sites of HIF-1alpha in mammalian genes were attempted. We used ChIP-Seq method, in which next gene sequencing technology and chromatin-