BSE-Seq of extreme pools of grain number per spike. Triticum aestivum

Based on the GNS values obtained in four environments, lines were re-arranged, and the two tails (each 30 lines) were selected to bulk extreme pools. Two pools (GNS-H and GNS-L) were bulked using euqal quantities (1 μg) of DNA from 30 individuals in the tails. Exon capture and sequencing of these four libraries (GNS-H and GNS-L) were performed by Bioacme Biotechnology Co., Ltd (Wuhan, China, http://www.whbioacme.com).