Transcriptome analysis of bone marrow in Phc2 knock out mice

This study investigated the biological function of Phc2 by Phc2 knock out mice. Polycomb group (PcG) proteins function as transcriptional repressors of target genes by mainly modulating histone methylation. The canonical Polycomb repressor complex 1 (PRC1), type of PcG proteins, recognizes and binds to H3K27me3 to sustain the transcriptional repression of a target gene. To investigate the function of canonical PRC1, We identified potential function of Phc2 that is component of the canonical PRC1 by Phc2 knock out mice. Phc2+/- (Phc2 heterozygote) mice with a C57BL/6 background were gifted by Dr. Kyoichi Isono (Wakayama Medical University). Wild type and Phc2 knock out mice were bred from Phc2 +/- mice.The bone marrow RNA extracts of Wild type and Phc2 knock out mice were used for transcriptome analysis.