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Comparison of gene expression quantification using a custom qPCR mix and Maxima® SYBR Green Master Mix.

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https://figshare.com/articles/dataset/_Comparison_of_gene_expression_quantification_using_a_custom_qPCR_mix_and_Maxima_174_SYBR_Green_Master_Mix_/305778
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cDNA (1 100 dilution) synthesized using a 20 µL reaction iScript™ cDNA synthesis and quantified in a 10 µL qPCR reaction using Maxima® SYBR Green Master Mix or custom made master mix. Values represent the fold difference between biofilm and planktonic population plus or minus standard deviation of 3 independent experiments. Values above 1 indicate up-regulation in biofilm, and below 1 indicates down-regulation. *Statistical differences (p<0.05) between custom and Maxima® SYBR Green Master Mix reactions. N/D – non detected.
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2012-05-21
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