The influence of turicibacter on constipation

To investigate the effects of different antibiotics (sisomicin sulfate, cefadroxil, ribostamycin sulfate) on gut microbe structure, 40 mice were randomly divided into four groups. The mice underwent a 7-day adaptation period. Sisomicin sulfate group was given 200 mg/kg sisomicin sulfate once per day for 7 days by oral gavage, cefadroxil group was given 130 mg/kg cefadroxil once per day for 7 days by oral gavage, ribostamycin sulfate group was given 400 mg/kg ribostamycin sulfate once per day for 7 days by oral gavage. The metagenomic data of their feces were measured.To investigate the effects of T. sanguinis in a loperamide-induced constipation model, 48 male SPF C57BL/6J mice (6 weeks old, weighing 19-21 g) were used. Animals were randomly divided into six groups:Control group: received 0.9% sodium chloride solution by oral gavage.Loperamide (LOP) group: received loperamide (10 mg/kg body weight) by oral gavage for 14 days to induce constipation.Cefadroxil intervention group: received 130 mg/kg cefadroxil by oral gavage once per day for 7 days, then received loperamide (10 mg/kg) plus cefadroxil for 14 days.Cefadroxil with natural recovery group: received 130 mg/kg cefadroxil by oral gavage once per day for 7 days, then received loperamide (10 mg/kg) for 14 days.T. sanguinis treatment group: received 130 mg/kg cefadroxil by oral gavage once per day for 7 days, then received loperamide (10 mg/kg) plus 1.0 x 10^9 CFU T. sanguinis once per day for 14 days.Positive drug control group: received 130 mg/kg cefadroxil by oral gavage once per day for 7 days, then received loperamide (10 mg/kg) plus 7 mg/kg phenolphthalein.The 16S data of their feces were measured.