Optimization of C11-BODIPY581/591 staining for lipid peroxidation in C. reinhardtii

The aim of this set of experiments was to optimize the C11-BODIPY581/591 staining conditions and to explore the capability of the dye to stain Chlamydomonas reinhardtii cells under various cell and dye concentrations and different staining procedures.
Conclusion:
The dye resulted to be applicable to a wide range of staining procedures thanks to its high stability inside the cellular membrane and its specificity of reaction with oxyl-radicals, moreover its low interference with cell autofluorescence makes it a reliable dye for algal staining. Accuri FCM calibration was periodically inspected following the manufacturer instructions during the entire work. Algal axenicity was repeatedly checked during the period of experiments. All experiments were performed at least three times as independent replicates