Collection of cryoSXT data to analyse changes in cellular morphology accompanying infection.

CryoSXT data was collected using a 25 nm zone plate from multiple uninfected cells or cells at early and late stages of infection across three independent replicates. Tiled X-ray projections (‘X-ray mosaics’) with a 66.2×66.2 μm field of view were collected at multiple areas on the sample grid to identify cells of interest. Tilt series were collected at perinuclear or peripheral regions of the cytoplasm within these cells and were processed to generate tomograms.