<b>Lipid metabolites affected by deficient autophagy antagonize the occurrence of autophagy through AMPK signaling in insects</b>

Study Purpose: To investigate the changes of lipid metabolites in Drosophila fat body due to premature or inhibited autophagySample Details: The larvae were collected for more than 20 mg in each sample from <i>WT</i>^{<em>1118</em>}, <i>DmAtg1</i>^{<em>Δ3D</em>},and<i> ppl-GAL4&gt;UAS-DmAtg1:DmAtg13</i> at wandering (W), white prepupa (WPP), and 3 h after white prepupa (3WPP) stages. Each set of treatments contained six biological replicates.Instrumentation and Data Processing: The <i>Drosophila</i> larvae in each sample were homogenized and extracted for lipids by 1 mL extract solution containing methyl tert-butyl ether and methanol in the volume ratio of 3:1 as well as international standard lipids. The supernatant was measured for metabolites by UPLC-MS/MS. The ultra performance liquid chromatography (UPLC, ExionLC AD), equipped with Thermo Accucore™ C30 column (i.d. 2.1 x 100 mm, 2.6 μm) and maintained at 45°C, was used for UPLC-MS/MS analysis (Wuhan Metware Biotechnology Co., Ltd., Wuhan, China).