Supporting data for "Torix Rickettsia are widespread in arthropods and reflect a neglected symbiosis"

<i>Rickettsia</i> are intracellular bacteria best known as the causative agents of human and animal diseases. Although these medically important <i>Rickettsia</i> are often transmitted via haematophagous arthropods, other <i>Rickettsia</i>, such as those in the Torix group, appear to reside exclusively in invertebrates and protists with no secondary vertebrate host. Importantly, little is known about the diversity or host range of Torix group <i>Rickettsia</i>.<br>This study describes the serendipitous discovery of <i>Rickettsia</i> amplicons in the Barcode of Life Data System (BOLD), a sequence database specifically designed for the curation of mtDNA barcodes. Out of 184,585 barcode sequences analysed, <i>Rickettsia</i> is observed in approximately 0.41% of barcode submissions and is more likely to be found than <i>Wolbachia</i> (0.17%). The Torix group of <i>Rickettsia</i> are shown to account for 95% of all unintended amplifications from the genus. A further targeted PCR screen of 1,612 individuals from 169 terrestrial and aquatic invertebrate species identified mostly Torix strains and supports the aquatic hot spot hypothesis for Torix infection. Furthermore, the analysis of 1,341 Sequence Read Archive (SRA) deposits indicates Torix infections represent a significant proportion of all <i>Rickettsia</i> symbioses found in arthropod genome projects.<br>This study supports a previous hypothesis which suggests Torix <i>Rickettsia</i> are overrepresented in aquatic insects. In addition, multiple methods reveal further putative hot spots of Torix <i>Rickettsia</i> infection; including in phloem-feeding bugs, parasitoid wasps, spiders, and vectors of disease. The unknown host effects and transmission strategies of these endosymbionts make these newly discovered associations important to inform future directions of investigation involving the understudied Torix <i>Rickettsia</i>.