Putative EHEC lipoproteins markedly enriched in size exclusion chromatography fraction F1-sSEC.

A: Categories. 1, verified lipoprotein (three listed out of >20); 2, conserved Sp II signal peptidase motif with a hydrophobic leader sequence; 3, tentative Sp II signal peptidase motif; *protein was also identified in a membrane phospholipid-enriched cell lysate fraction derived from iodixanol density gradient centrifugation experiments.B: Protein annotations from the databases EcoCyc or E. coli O157:H7 strain EDL933; gene name (in parentheses): listed only in the UniProt.C: Prediction of subcellular localization and other annotation features such as multi-protein complex (MPC) with 2 or more subunits based database entries; L: lipoprotein; IM, inner membrane; OM, outer membrane; no hydr/hydr LS (presence/absence of hydrophobic leader sequence N-terminal to Sp II motif).D: Subcellular localization prediction based on PSORTb assignment; unkn (unknown); PP (periplasm); CY (cytoplasm); mscl (multiple localizations).E: Average APEXi protein abundance score (n = 12) for protein in size exclusion chromatography fractions F1-sSEC (F1–13, Mr >280 kDa) where membrane proteins were enriched and F2-sSEC (F14–26, Mr 280-80 kDa); *score from fraction F3-sSEC if score was ‘0’ in F2-sSEC.F: Signal peptidase II motif surrounding the lipid-anchored cysteine residue; tentative motif assignment for category 3 proteins. The number denotes the Cys amino acid position in the protein sequence provided in the E. coli O157:H7 EDL933 database.G: Δseq: likely incorrect prediction of translational start site in E. coli O157:H7 EDL933 database (number of amino acids represents the respective shift based on our corrections); s. Z2148, Z1474 etc.: high sequence similarity among genes encoded by prophage regions.